What is the optimum pH for the protease enzyme?
Optimum pH and stability of the purified protease The enzyme was found to be highly active in the pH ranges from 7 to 12, with an optimum pH 8 (Figure 2A).
What is aspartic protease?
Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate residues for catalysis of their peptide substrates. In general, they have two highly conserved aspartates in the active site and are optimally active at acidic pH.
What is an active protease with a low pH optimum?
Pepsin. a protease enzyme with a low pH optimum.
Is pepsin an aspartic protease?
The best known sources of aspartic proteases are stomach (for pepsin, gastricsin, and chymosin), lysosomes (for cathepsins D and E), kidney (for renin), yeast granules, and fungi (for secreted proteases such as rhizopuspepsin, penicillopepsin, and endothiapepsin).
What pH is optimum lipase?
The purified lipase was observed to be active in the pH ranging from 7 to 9 and optimum pH for lipase action was observed to be 8 (Figure 4B).
How does serine protease work?
Serine proteases catalyze peptide bond hydrolysis in two sequential steps. In the first (acylation) reaction, the nucleophilic serine attacks the substrate scissile bond, forming first a tetrahedral intermediate and then a covalent acyl-enzyme with release of the C-terminal fragment.
What are the optimum conditions for protease?
The optimum temperature for the protease activity was observed at 40° C with substantial activity between 30 and 50° C (Figure 2). At 50 and 60° C the activity decreased to 97% and 85%, respectively.
What is the optimum temperature for protease?
The optimum activity of crude protease extract was found at pH 7.0 and 50°C. The crude protease enzyme was highly stable over a wide pH range of 4.0-11.0 and showed high stability at temperatures below 40°C.