What is the readout device of spectronic 20d?

What is the readout device of spectronic 20d?

The Bausch & Lomb Spectronic 20 colorimeter uses a diffraction grating monochromator combined with a system for the detection, amplification, and measurement of light wavelengths in the 340 nm to 950 nm range.

What is the spectrophotometer used for?

Spectrophotometry is a standard and inexpensive technique to measure light absorption or the amount of chemicals in a solution. It uses a light beam which passes through the sample, and each compound in the solution absorbs or transmits light over a certain wavelength.

What is laboratory spectrophotometer?

Spectrophotometer is a device used to measure the intensity of light as a function of the color of light. In clinical laboratories, spectrophotometers are used for many applications such as quantitative analyses, kinetics, spectrum scanning and multiple components DNA/protein testing.

How do you use a digital spectrophotometer?


  1. Select a blank cuvette and place it in the spectrophotometer. Close the lid.
  2. Click on 0 ABS 100%T button, the instrument now reads 0.00000 A.
  3. Choose a solution with known concentration and measure the absorbance between the wavelengths 350 nm to 700 nm.
  4. Record the wavelength at the maximum absorbance value.

Why is Isosbestic point importance?

Isosbestic points are also used in clinical chemistry, as a quality assurance method, to verify the accuracy in the wavelength of a spectrophotometer. This is done by measuring the spectra of a standard substance at two different pH conditions (above and below the pKa of the substance).

What are the 2 types of spectrophotometer?

There are two major classes of devices: single-beam and double-beam. A double-beam spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample.