What is the readout device of spectronic 20d?
The Bausch & Lomb Spectronic 20 colorimeter uses a diffraction grating monochromator combined with a system for the detection, amplification, and measurement of light wavelengths in the 340 nm to 950 nm range.
What is the spectrophotometer used for?
Spectrophotometry is a standard and inexpensive technique to measure light absorption or the amount of chemicals in a solution. It uses a light beam which passes through the sample, and each compound in the solution absorbs or transmits light over a certain wavelength.
What is laboratory spectrophotometer?
Spectrophotometer is a device used to measure the intensity of light as a function of the color of light. In clinical laboratories, spectrophotometers are used for many applications such as quantitative analyses, kinetics, spectrum scanning and multiple components DNA/protein testing.
How do you use a digital spectrophotometer?
Procedure:
- Select a blank cuvette and place it in the spectrophotometer. Close the lid.
- Click on 0 ABS 100%T button, the instrument now reads 0.00000 A.
- Choose a solution with known concentration and measure the absorbance between the wavelengths 350 nm to 700 nm.
- Record the wavelength at the maximum absorbance value.
Why is Isosbestic point importance?
Isosbestic points are also used in clinical chemistry, as a quality assurance method, to verify the accuracy in the wavelength of a spectrophotometer. This is done by measuring the spectra of a standard substance at two different pH conditions (above and below the pKa of the substance).
What are the 2 types of spectrophotometer?
There are two major classes of devices: single-beam and double-beam. A double-beam spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample.