What is lymphoprep?

What is lymphoprep?

Lymphoprep™ is a density gradient medium recommended for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density.

What is lymphoprep made of?

Art.No.: 1114544

Quantity 1×250 ml
Product description Lymphoprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of pure lymphocyte suspensions. The solution contains sodium diatrizoate and polysaccharide.
Density 1.077 ± 0.001 g/ml
Composition 9.1% (w/v) Sodium Diatrizoate, 5.7% (w/v) Polysaccharide

What is density gradient reagent?

Density Gradient Reagents In density gradient centrifugation, the reagent is a product used to assist in isolation or separation of the cells. Not only can these products speed up the process, they can also increase the purity and throughput.

What is media density?

Density gradient centrifugation is commonly used for the isolation of specific cell populations from whole blood. To isolate mononuclear cells (MNCs) from peripheral blood, cord blood and bone marrow, it is recommended to use media with a density of 1.077 g/mL, such as Lymphoprep™ or Ficoll-Paque™.

What is Ficoll Hypaque?

Ficoll-Hypaque is a solution of sodium diatrizoate (a dense, iodine-containing molecule) and Ficoll (a polysaccharide) with a density (1.083 g/cc) that falls between that of neutrophils and that of the mononuclear cells.

Is lymphoprep light sensitive?

Protect product from light. Prolonged exposure to direct sunlight leads to release of iodinine from the sodium diatrizoate molecule. This effect is negligible when working with this solution on a day-to-day basis.

What is percoll used for?

Percoll is a tool for more efficient density separation in biochemistry that was first formulated by Pertoft and colleagues. It is used for the isolation of cells, organelles, and/or viruses by density centrifugation.

What is the viscosity of cell culture media?

CFD studies of in vitro systems typically model culture media as water at room temperature, where density and viscosity is assumed to be anywhere between 0.998-1000 kg/m3 and 0.8-1 mPa.