What is hha1?
Thermo Scientific HhaI restriction enzyme recognizes GCG^C sites and cuts best at 37°C in Tango buffer. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions.
Is Crispr a restriction enzyme?
CRISPR is not a restriction enzyme.
What is the key to the high specificity of restriction enzymes?
Methylation of DNA Strands Methylation of DNA is a major factor that affects the specificity of restriction endonucleases. DNA isolated from strains of bacteria expressing methylases such as Dam or Dcm may be resistant to cleavage by endonucleases as the recognition sites are methylated. Plasmid DNA isolated from E.
What is HhaI restriction endonuclease?
An E. coli strain that carries the cloned HhaI modification gene from Haemophilus haemolyticus (ATCC 10014). One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by HhaI restriction endonuclease.
What is the HhaI restriction enzyme used for?
Thermo Scientific HhaI restriction enzyme recognizes GCG^C sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
What is meant by one unit of enzyme protection?
One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by HhaI restriction endonuclease.
What is HhaI 1 unit?
An E. coli strain that carries the HhaI gene from Haemophilus haemolyticus (ATCC 10014). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. HinP1I is an isoschizomer of HhaI.