What is ABI PCR?

The ABI PRISM 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5′ nuclease assay.

What is a 7500 Fast real-time PCR system?

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

How much is a qPCR machine?

Roche’s qPCR instruments run from about $25,000 in the United States for the LightCycler 2.0 to nearly $140,000 for the ultra-high-throughput LightCycler 1536.

How is qPCR different from PCR?

The main difference between PCR and qPCR is that PCR is a qualitative technique whereas qPCR is a quantitative technique. PCR allows reading the result as “presence or absence’. But in qPCR, the amount of DNA amplified in each cycle are quantified.

How does a qPCR machine work?

A quantitative PCR instrument is a machine that amplifies and detects DNA. It combines the functions of a thermal cycler and a fluorimeter, enabling the process of quantitative PCR.

What is the 7500 Fast?

The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. Faster ramp rates enable rapid results without compromising extension times or assay quality.

Is qPCR expensive?

As you probably know, qPCR reagents are not exactly cheap. qPCR thermocyclers with 384-well blocks tend to be more expensive than those with 96-well blocks. 60 EUR (or 167.86 USD) using a 384-well qPCR cycler – 384 reactions (0.36 EUR / 0.44 USD per reaction)

How much does qPCR Cost per sample?

Gene Expression Analysis (QPCR) Pricing

Number of samples	1	8
Cost per sample	405	99
3 Primer Sets	485	950
Cost per sample	485	119
4 Primer Sets	570	1125

What is the main advantage of qPCR over the conventional PCR?

The main advantages of qPCR are that it provides fast and high-throughput detection and quantification of target DNA sequences in different matrices. The lower time of amplification is facilitated by the simultaneous amplification and visualization of newly formed DNA amplicons.

Is qPCR the same as real-time PCR?

Quantitative PCR (qPCR), also called real-time PCR or quantitative real-time PCR, is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction.

What is qPCR and how does it work?

qPCR works by amplifying target DNA to levels in which a detectable signal can be observed. The reaction is prepared by mixing a small amount of a sample (e.g., saliva or urine) with the building blocks needed to make copies of the DNA sequence. What sets qPCR apart from older methods is that the reaction mixture also contains fluorescent probes, or DNA binding dyes.

What is the function of a PCR machine?

The human immunodeficiency virus (or HIV ),is a difficult target to find and eradicate.

Some disease organisms,such as that for tuberculosis,are difficult to sample from patients and slow to be grown in the laboratory.

The spread of a disease organism through populations of domestic or wild animals can be monitored by PCR testing.

What is an ABI machine?

Place the blood pressure cuff on the right upper arm.

Place the Doppler probe at the antecubital fossa (to find the right brachial artery) or the thumb side of the wrist (to find the radial artery).

Brace your ring finger or pinky on the patient’s arm or bed to steady your hand and avoid artifacts.

What is quantitative PCR?

Quantitative PCR (qPCR) is used to detect, characterize and quantify nucleic acids for numerous applications. Commonly, in RT-qPCR, RNA transcripts are quantified by reverse transcribing them into cDNA first, as described above and then qPCR is subsequently carried out.