What is a type 1 NKT?

What is a type 1 NKT?

Type 1 NKTs are an evolutionarily conserved subset of innate lymphocytes characterized by expression of invariant TCR α-chain Vα24-Jα18 and reactivity to glycolipids presented by the HLA class-I-like molecule CD1d (7).

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How are lentiviral supernatants generated from NKTs?

Lentiviral supernatants were generated from 293T cells transfected with relevant lentiviral construct(s), pMD2.G, and delta 8.2. Proliferation and apoptosis assays NKTs were labeled with CellTrace Violet (CTV; Thermo Fisher, Waltham, MA) and stimulated with αGalCer-pulsed B-8–2 cells.