What does the Kirby-Bauer disc diffusion method tell you?
The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.
What information does the Kirby-Bauer method provide?
The Kirby-Bauer or disk diffusion test is used to determine if an organism is susceptible or resistant to a selection of antimicrobial agents. When the test is run in a very specific manner, it can even be used to determine how susceptible an organism is to a specific antimicrobic.
What is the purpose of the disk diffusion test?
In diagnostic laboratories, the disk diffusion test is used to determine the susceptibility of clinical isolates of bacteria to different antibiotics.
How is the information from a Kirby-Bauer disk diffusion test used for the recommendation of the clinical use of an antimicrobial drug?
The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic susceptibility test in determining what choice of antibiotics should be used when treating an infection. This method relies on the inhibition of bacterial growth measured under standard conditions.
How can we utilize the results from a Kirby-Bauer assay to determine the effectiveness of an antimicrobial drug in a patient?
The Kirby-Bauer disk diffusion test has long been used as a starting point for determining the susceptibility of specific microbes to various antimicrobial drugs. The Kirby-Bauer assay starts with a Mueller-Hinton agar plate on which a confluent lawn is inoculated with a patient’s isolated bacterial pathogen.
What is the principle involved in disk-diffusion technique?
PRINCIPLE. This method is based on the principle that antibiotic-impregnated disk, placed on agar previously inoculated with the test bacterium, pick-up moisture and the antibiotic diffuse radially outward through the agar medium producing an antibiotic concentration gradient.
What is the principle of the agar disc diffusion method?
This method is based on the principle that antibiotic-impregnated disk, placed on agar previously inoculated with the test bacterium, pick-up moisture and the antibiotic diffuse radially outward through the agar medium producing an antibiotic concentration gradient.