What does Dectin 1 recognize?
Dectin-1 specifically recognizes soluble and particulate β(1→3)- and/or β(1→6)-linked glucans27; these are carbohydrate polymers with immunomodulatory activity that are found mainly in the cell walls of fungi, but also in plants and some bacteria.
Is Dectin-1 a pattern recognition receptor?
Dectin-1 is a mainly myeloid-cell-expressed NK-cell-receptor-like C-type lectin that functions as a transmembrane pattern-recognition receptor through its ability to bind β-glucan carbohydrates. Dectin-1 can recognize several fungal pathogens and might play a role in the innate response to these organisms.
How is zymosan recognized by macrophages?
Zymosan is also recognized by Dectin-1, a phagocytic receptor expressed on macrophages and dendritic cells, which collaborates with TLR2 and TLR6 enhancing the immune responses triggered by the recognition of Zymosan by each receptor [2].
How do you opsonize zymosan?
Opsonized zymosan, labeled with the fluorophore Alexa Fluor 594, or opsonized latex beads (3 µm particle size) were used as a stimulus. The zymosan particles and latex beads were opsonized by incubation with murine serum for 20 min at 37°C, at a ratio of 1 ml serum per 10 mg particles ( 38 ).
What is the function of zymosan in Saccharomyces cerevisiae?
Zymosan is a component of the cell wall of the yeast Saccharomyces cerevisiae which activates macrophages trough the engagement of TLR2 or Dectin-1 receptors (Romao et al., 2013; Sanjuan et al., 2007). We and others have shown the recruitment of LC3B to zymosan-containing phagosomes.
How is zymosan recognized by Dectin-1?
Zymosan is also recognized by Dectin-1, a phagocytic receptor expressed on macrophages and dendritic cells, which collaborates with TLR2 and TLR6 enhancing the immune responses triggered by the recognition of Zymosan by each receptor [2]. 1. Ozinsky A. et al., 2000.