What is DMEM GlutaMAX?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Does DMEM have phosphate?
DMEM, high glucose, no phosphates.
What is the function of DMEM?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is DMEM 10?
Dulbecco’s modified Eagle’s medium (DMEM; GIBCO/Invitrogen) 10% normal calf serum. 1% penicillin/streptomycin (Cellgro) 1% L-glutamine (Cellgro) Filter-sterilize through a 0.22-μm filter.
What is the composition of DMEM media?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
Does DMEM contain calcium?
The complete formulation is available. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium….This DMEM is modified as follows:
| With | Without |
|---|---|
| • High Glucose | • L-glutamine |
| • Phenol Red | • Sodium Pyruvate |
| • HEPES | |
| • Calcium |
What is the difference between DMEM and glutamax?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
What is GIBCO DMEM?
Gibco DMEM delivers superior quality, consistency, and control for your mammalian cell culture. We offer a variety of Gibco DMEM formulations to support a wide range of cell culture applications.
What is the composition of glutamax media?
Gibco ™ GlutaMAX media are standard cell culture media that contain a stabilized form of L-glutamine, the dipeptide L-alanyl-L-glutamine, that prevents degradation and ammonia buildup even during long-term culture (Figures 2 and 3).
What is the stability of GIBCO media?
Media stability keeps cells healthier. Gibco ™ GlutaMAX media are standard cell culture media that contain a stabilized form of L-glutamine, the dipeptide L-alanyl-L-glutamine, that prevents degradation and ammonia buildup even during long-term culture (Figures 2 and 3).