What is dT oligo?
Description. Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.
Why is Oligo dT an effective primer for reverse transcriptase?
Oligo(dT) primers amplify only mRNAs containing a poly(A) tail, since that is where the primer binds to promote reverse transcription. Random primers amplify most RNA species, including degraded RNA and viral genomes.
How do oligo dT beads work?
The use of Dynabeads® Oligo (dT)25 relies on base pairing between the poly A tail of messenger RNA and the oligo dT sequences bound to the surface of the beads. The oligo dT bound to the bead surface can be used to both capture the mRNA and act as a primer for reverse transcriptase during first strand cDNA synthesis.
What is a poly dT primer?
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of >=50°C, oligo(dT)20 primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA. Quality Control: This product is qualified in a first-strand cDNA synthesis reaction.
What are oligo primers?
Researchers use oligos that are anywhere between 20-35 bases long called primers to start copying or amplifying. This DNA primer is usually custom designed to match the target sequence of DNA for copying.
What is the purpose of the oligo dT primers when synthesizing cDNA?
First-strand synthesis of cDNA utilizes either oligo(dT), random primers, or a combination of these strategies to prime the reverse transcription reaction. Priming a reaction with oligo(dT) initiates the synthesis preferentially at the 3′ end of the RNA fragment.
Why do we use random hexamers in cDNA synthesis?
Random Hexamer Primers are commonly used for priming single-stranded DNA or RNA for extension by DNA polymerases or reverse transcriptases. During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA.